Hajizadeh, E. International Journal of Pediatrics , 2 2. International Journal of Pediatrics , 2, 2. International Journal of Pediatrics , ; 2 2. Abstract Background: Pancreatic islet transplantation has emerged as a promising treatment for type I diabetes. However, its efficacy is severely hampered due to poor islet engraftment and revascularization, which have been resulted to partially loss of transplanted islets.
It has been shown that local delivery of vascular endothelial growth factor VEGF could accelerate transplanted islet revascularization, although permanent high level of VEGF may lead to undesirable side effects. In this study we investigated the effects of conditional cell-based delivery of VEGF through collagen-fibrin hydrogel on transplanted islet function and revascularization. After co-transplantation of these cells and mouse isolated islets through collagen-fibrin hydrogel in the omental pouch of diabetic nude mice, the blood glucose, body weight, glucose tolerance and serum C-peptide was measured after 28 days.
For measurement of revascularization, immunohistoflourscence examination was done and micro vessel density MVD and vessel size have been measured.
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This notable difference in blood sugar of transplant groups, was also reflected in their weight gain. This result can be used to improve the outcome of clinical islet transplantation. Low-volume hydrodynamic gene delivery to the rat liver via an isolated segment of the inferior vena cava: efficiency, cardiovascular response and intrahepatic vascular dynamics.
Minimally invasive and selective hydrodynamic gene therapy of liver segments in the pig and human. Hydrodynamic gene delivery to the pig liver via an isolated segment of the inferior vena cava. Preferential delivery of the Sleeping Beauty transposon system to livers of mice by hydrodynamic injection. Cardiovascular function following acute volume overload for hydrodynamic gene delivery to the liver.
Improved anti-cancer effect of interferon gene transfer by sustained expression using CpG-reduced plasmid DNA. The administration of naked plasmid DNA into the liver induces antitumor innate immunity in a murine liver metastasis model. RNAi therapeutics: Principles, prospects and challenges. Systemic siRNA delivery via hydrodynamic intravascular injection. Nonviral gene delivery: What we know and what is next.
Pig liver gene therapy by noninvasive interventionist catheterism. Naked plasmid DNA transfer to the porcine liver using rapid injection with large volume.
Materials and Methods
Hydrodynamics-based gene delivery of naked DNA encoding fetal liver kinase-1 gene effectively suppresses the growth of pre-existing tumors. Mechanism of plasmid delivery by hydrodynamic tail vein injection. Hepatocyte uptake of various molecules. Jaichandran Steven T. Yap Adrian B. Genetic immunization for antibody generation in research animals by intravenous delivery of plasmid DNA.
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Hydrodynamics based transfection in normal and fibrotic rats. Novel delivery methods for treatment of viral hepatitis: an update. Reducing the immunostimulatory activity of CpG-containing plasmid DNA vectors for non-viral gene therapy. Gene Therapy Progress and Prospects: Electroporation and other physical methods. Unlocking the potential of the human genome with RNA interference. Gene therapy of liver diseases. Liver-directed gene therapy for dyslipidemia and diabetes.
Hydroporation as the mechanism of hydrodynamic delivery.
Interleukin genetic administration suppressed metastatic liver tumor unsusceptible to CTL. Insulin expression in livers of diabetic mice mediated by hydrodynamics-based administration. Interleukin IL and IL genetic transfer synergistically augments therapeutic antitumor immunity and promotes regression of metastatic lymphoma. Carol H.
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Miao Xin Ye Arthur R. Hydrodynamic delivery of DNA. Normalization of growth in hypophysectomized mice using hydrodynamic transfer of the human growth hormone gene. Progress and prospects: naked DNA gene transfer and therapy.
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